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CSF coccidioides complement fixation test

Coccidioides antibody test - spinal fluid

CSF coccidioides complement fixation is a test that checks for infection due to the fungus Coccidioides spp. in the cerebrospinal (CSF) fluid. This is the fluid surrounding the brain and spinal cord. The name of this infection is coccidioidomycosis, or valley fever. When the infection involves the covering of the brain and spinal cord (the meninges), it is called coccidioidal meningitis.

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How the Test is Performed

A sample of spinal fluid is needed for this test. The sample is usually obtained by lumbar puncture (spinal tap).

The sample is sent to a lab. There, it is examined for Coccidioides antibodies using a lab method called complement fixation. This technique checks if your body has produced substances called antibodies to a specific foreign substance (antigen), in this case Coccidioides.

Antibodies are specialized proteins that defend your body against bacteria, viruses, and fungi. If the antibodies are present, they stick, or fix themselves, to the antigen. This is why the test is called fixation.

How to Prepare for the Test

Follow your health care provider's instructions on how to prepare for the test. Expect to be in the hospital for several hours afterward.

How the Test Will Feel

During the lumbar puncture procedure:

• You lie on your side with knees pulled up toward your chest and chin tucked downward. Or, you sit up, but you are bent forward.
• After your back is cleaned, the doctor injects a local numbing medicine (anesthetic) into your lower spine.
• A spinal needle is inserted, usually into the lower back area.
• Once the needle is properly positioned, CSF pressure is measured, and a sample is collected.
• The needle is removed, the area is cleaned, and a bandage is placed over the needle site.
• You are taken to a recovery area where you rest for several hours to prevent any CSF leakage.

Why the Test is Performed

This test checks if your central nervous system has an active infection from Coccidioides.

Normal Results

The absence of fungus (a negative test) is normal.

What Abnormal Results Mean

If the test is positive for fungus, there may be an active infection in the central nervous system.

An abnormal spinal fluid test means that the central nervous system is infected. During the early stage of an illness, few antibodies may be detected. Antibody production increases during the course of an infection. For this reason, this test may be repeated several weeks after the first test.

Risks

Risks of lumbar puncture include:

• Bleeding into the spinal canal
• Discomfort during the test
• Headache after the test
• Hypersensitivity (allergic) reaction to the anesthetic
• Infection introduced by the needle going through the skin
• Damage to the nerves in the spinal cord, occurs more commonly if the person moves during the test

Related Information

References

Aoyagi K, Ashihara Y, Kasahara Y. Immunoassays and immunochemistry. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 24th ed. Philadelphia, PA: Elsevier; 2022:chap 45.

Galgiani JN. Coccidioidomycosis (Coccidioides species). In: Blaser MJ, Cohen JI, Holland SM, et al, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 10th ed. Philadelphia, PA: Elsevier; 2026:chap 271.

Thompson GR, Miceli MH. Endemic mycoses. In: Goldman L, Cooney KA, eds. Goldman-Cecil Medicine. 27th ed. Philadelphia, PA: Elsevier; 2024:chap 308.

Review Date: 5/12/2025  

Reviewed By: Jatin M. Vyas, MD, PhD, Roy and Diana Vagelos Professor in Medicine, Columbia University Vagelos College of Physicians and Surgeons, Division of Infectious Diseases, Department of Medicine, New York, NY. Also reviewed by David C. Dugdale, MD, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.

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